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cspD [2019-09-04 09:33:52]
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cspD [2019-09-04 09:33:52]

cold shock protein
locus
BSU_21930
pI
4.31
mw
7.17 kDa
protein length
66 aa Sequence Blast
gene length
201 bp Sequence Blast
function
RNA chaperone
product
cold shock protein
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.2|RNA chaperones]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.5|Cold stress proteins]

    • Gene

    Coordinates
    2,307,905 2,308,105

    The protein

    Paralogous protein(s)

  • [protein|16E048FE5AF76CC771DEBBA8CB56B75A5414B3A2|CspB], [protein|D453E1E7D4256B56AFB2149731E08B104D59431B|CspC]

    • Structure

  • [PDB|1C9O] (from ''B. caldolyticus'', 86% identity) [Pubmed|10736231]

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]
  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|cspD]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • GP2614 ([gene|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|cspD]::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • MGNA-A874 (cspD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/874 NBRP B. subtilis, Japan]
  • BKE21930 ([gene|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|cspD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGCTTAATTCCTCCT, downstream forward: _UP4_TAAACTCAATACATGATGAT
  • BKK21930 ([gene|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|cspD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGCTTAATTCCTCCT, downstream forward: _UP4_TAAACTCAATACATGATGAT

    • Expression vectors

  • pGP2164: expression of ''cspD''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • pGP2165: expression of ''cspD''-Strep by [SW|pGP380] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 12171653,12838604

    • Original publications

  • 12399512,11752341,11717297,9920884,12427936,11591689,9379903,17981983,15378759,10736231